Thermal inactivation of p-chloromer-curibenzoate-dithiothreitol-treated myosin ATPase.
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چکیده
منابع مشابه
Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 0.0025 M and 0.005 M, pH between 7.0 and 8.0, and incubation at 37 degrees C. Concentrations over 0.01 M, pH values greater than 8.0, and an incubation temperature over 4...
متن کاملInactivation of pea leaf phosphofructokinase by light and dithiothreitol.
When intact pea (Pisum sativum L.) plants are illuminated, the glycolytic enzyme phosphofructokinase is inactivated. In crude extracts the enzyme is inhibited by dithiothreitol. It would seem that this cytoplasmic enzyme, like glucose-6-P dehydrogenase, is light-inactivated when the enzymes of photosynthetic carbon metabolism are light-activated.
متن کاملEvaluation of dithiothreitol ( DTT ) for inactivation of IgM antibodies 1
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0, and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubation temperature over 40'C result...
متن کاملMyosin heavy chain isoform composition influences the susceptibility of actin-activated S1 ATPase and myofibrillar ATPase to pH inactivation.
The objectives of this study were to determine the influence of pH and MyHC isoforms on myofibrillar and actin-activated myosin subfragment 1 (S1) ATPase activity and the protective effect of actin. Red (RST) semitendinosus and white (WST) semitendinosus myofibrils were incubated at pH 7, 6, or 5.5 with 0 or 2mM ATP. RST and WST S1 isolates were incubated at pH 7, 6, or 5.5 in the presence or a...
متن کاملPhysical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN. The analysis was done by dividing the p-CBN sa...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1976
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.40.633